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. 2019 Apr 18;13:391. doi: 10.3389/fnins.2019.00391

Figure 2.

Figure 2

Effects of SG2A on body weight change and anxiety/depression-like behaviors in mice with corticosterone pellet implantation (CORTI) and in sham-operated mice. (A) Experimental scheme for SG2A administrations and behavioral tests after CORTI. SG2A or vehicle (Veh) was administered to CORTI or sham-operated mice for 8 consecutive days, during which body weight measurement, the sucrose preference test (SPT), elevated plus maze test (EPMT), open field test (OFT), and tail suspension test (TST) were conducted. After all behavioral tests, blood and brain samples were collected for determination of CORT levels, neurogenesis in the dentate gyrus of the hippocampus, and tryptophan hydroxylase (TPH) protein levels in the dorsal raphe nucleus. (B,C) Body weight changes during 3 weeks of CORT implantation and after SG2A administration [arrow in panel (B)] for 7 days. Effects of SG2A on sucrose preference (D), total fluid consumption (E), times in the open-arm in the EPMT (F) and in the center in the OFT (G), and immobility of mice in the TST (H). (I) Immunohistochemistry of BrdU (red), doublecortin [(DCX) green] and DAPI (blue) (left) and mean number of BrdU+DCX+ cells (right) in the DG of the hippocampus (5–6 slices were counted per mouse). (J) Western blot for tryptophan hydroxylase [(TPH) top] and TPH levels in the DRN (bottom). Data are presented as means ± SEMs; ∗P < 0.05 and ∗∗P < 0.01 vs. Sham-Veh, #P < 0.05 and ##P < 0.01 vs. CORTI-Veh. Numbers in parentheses indicated the numbers of animals used for each group. Scale bar, 50 μm.