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. 2019 Apr 18;10:850. doi: 10.3389/fimmu.2019.00850

Figure 3.

Figure 3

TonEBP suppresses the macrophage M2 phenotype by downregulating HO-1. (A,B) Differentiated THP-1 (A) and RAW264.7 (B) cells were transfected with scrambled [Scr (-)], TonEBP-targeting, or HO-targeting siRNA in the combinations indicated at the bottom of the panels for 24 h. The concentration of total siRNA was equalized by adjusting the concentration of Scr (-) siRNA. Transfected cells were then treated with LPS (100 ng/ml) for 12 h, and expression of mRNA encoding IL-10, Arg-1, and CD206 was measured by quantitative RT-PCR. (C,D) RAW264.7 cells transfected with Scr (-) or TonEBP-targeting siRNA were treated for 12 h with LPS in the presence of ZnPP (20 μM), CoPP (5 μM), or vehicle (-). The abundance of mRNA encoding IL-10, Arg-1, and CD206 was measured by quantitative RT-PCR. Two-way ANOVA with Tukey's post-hoc test was used for multiple comparisons. Different letters indicate statistical differences at P < 0.05. Data (mean + SD) were from three independent experiments (n = 3) each with more than three replicates. AU, arbitrary units.