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. Author manuscript; available in PMC: 2019 Apr 25.
Published in final edited form as: Chembiochem. 2014 Sep 15;15(16):2435–2442. doi: 10.1002/cbic.201402321

Figure 7.

Figure 7.

A) Time dependent inhibition by MDP. eEF-2K was incubated with MDP (500 μM) for different amounts of time (0, 1, 5, 10, 20, 30, 45 and 60 min). The E+I mix was diluted 50 fold into the assay reaction. Formation of the E•I* complex against incubation time was fitted using Equation 1. The kobs for the formation of E•I* complex is 0.0031 ± 0.0002 s−1. B) Recovery of enzyme activity against MDP. The enzyme was first incubated with MDP (500 μM) for 60 min, then diluted 50-fold and incubated for different timepoints, before starting the assay. The loss of the E•I* complex against incubation time after dilution was fitted using Equation 2. The kobs for the loss of the E•I* complex is 0.00056 ± 0.00003 s−1.