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. 2019 Apr 24;9:6491. doi: 10.1038/s41598-019-43032-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cells were grown overnight in 4% glucose media. In the morning, cells were diluted and grown for an additional 3 hours in 4% glucose. After measuring the basal ADH2 expression (t = 0), cells were washed three times with DDW and resuspended in media containing either ethanol (2%) or sodium acetate pH6 (2%). ADH2 expression was determined after 3 hours. Rate of expression is normalized against WT cells in ethanol media. N = 3. Error bars are one standard deviation.