Figure 3.

(a) Cells were treated as in Fig. 2 (b). Cells were grown overnight in the indicated media (without dilution). 0.8OD was harvested and ADH activity determined. Activity is in mU/OD₆₀₀. N = 3. Error bars are one standard deviation. (c) Cells [WT or Δady2Δfps1Δjen1] were grown overnight in 4% glucose. In the morning, cells were diluted and grown for an additional 3 hours in 4% glucose media. After measuring basal YRO2 expression, sodium acetate ph5.6 was added to 2% and YRO2 expression determined after 3 hours. Rate of expression is normalized against WT cells in acetate media. N = 3. Error bars are one standard deviation. (c) Cells were grown for 24 hours in 5 ml of the indicated media (without dilution) and YRO2 expression measured. Expression is normalized against BY4741 in glycerol +2% acetate medium. N = 3. Error bars are one standard deviation. (d) Cells were grown as in (b) Cells were imaged in 4% glucose media and 45 minutes following addition of acetate (pH 5.6) to 2%. (e) Quantification of d as described in Material and Methods. (f,g) Cells were grown for 24 hours in 5 ml of the indicated media (without dilution) and YRO2 expression measured. Expression is normalized against WT in acetate medium. N = 3. Error bars are one standard deviation.