Figure 3.

Short-circuit current measurements under 0 Chloride conditions in WT and CF Human Bronchial Epithelial primary cells. Experiments were done in a solution with 0 Cl⁻ and 25 mM HCO₃⁻, bubbled with 95% O₂ and 5% CO₂ at the apical and basal faces. Short-circuit current variation were induced by successive addition of amiloride (100 µM), Forskolin/IBMX (10 µM/100 µM), VX770 (10 µM), Inh-172 (5 µM). (A) Representative experiment from WT Human Bronchial Epithelial (HBE) primary cells. (B) Representative experiment from CF HBE primary cells (N1303K/4005 + 1G > A). (C) Summary of results obtained from 3 WT subjects (n = 3; 14 filters) (grey bars) and 1 CF patient (N1303K/4005 + 1G > A; 2 independent experiments; 7 filters) (black bars). ΔForsk/IBMX: 5.4 ± 0.8 µA/cm² in WT HBE vs. 0.4 ± 0.1 /cm² in CF HBE; p = 0.012. ΔInh-172: −4.9 ± 0.97 µA/cm² in WT HBE vs. −0.1 ± 0.05 µA/cm² in F508del HBE; p = 0.02. Data are presented as mean ± SEM. Statistical significance from unpaired nonparametric Wilcoxon test. *p < 0.05.