Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 24;9:6516. doi: 10.1038/s41598-019-42751-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Airway surface liquid pH measurements upon SLC26A4 inhibition in WT and F508del bronchial epithelia. CFBE41o⁻ cells and Human Bronchial Epithelial (HBE) primary cells were incubated with DMSO vehicle (empty bars) or specific pendrin inhibitor A01 (filled bars) for 6 h. ASL pH was measured after apical addition of 50 µL Ringer’s solution (25 mM HCO₃⁻, 5% CO₂). DMSO induces a slight ASL acidification in both WT and F508del cells reaching a decrease of 0.2 pH units after 6 h incubation. (A) WT CFBE41o⁻ cells. pH = 7.20 ± 0.04 (DMSO) vs. pH = 7.14 ± 0.03 (A01), p = 0.008. (B) F508del CFBE41o⁻ cells. pH = 7.03 ± 0.03 (DMSO) vs. pH = 7.06 ± 0.04 (A01), NS. (C) WT HBE primary cells. pH = 7.19 ± 0.01 (DMSO) vs. pH = 7.08 ± 0.02 (A01), p = 0.04. (D) F508del HBE primary cells. pH = 6.99 ± 0.03 (DMSO) vs. pH = 7.02 ± 0.03 (A01), NS. For all conditions, n = 3 in triplicate. Data are presented as mean ± SEM. Statistical significance from unpaired nonparametric Wilcoxon test. *p < 0.05; **p < 0.01; NS: non significant.