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. 2019 Apr 24;9:6516. doi: 10.1038/s41598-019-42751-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clearance of S. aureus CIP 76.25 in airway surface liquid of WT and F508del bronchial epithelia. Epithelia were apically infected with S. aureus CIP 76.25 (3,000 CFU/mL). ASL was collected after 2 hours and plated on Petri dishes to count the surviving bacteria expressed as % from the inoculum. (A) CFBE41o⁻ cells. % of surviving bacteria at 2 h: 95 ± 9% in WT vs. 116 ± 11% in F508del, p = 0.04. For all conditions, n = 5 in triplicate. (B) Human Bronchial Epithelial (HBE) primary cells. % of surviving bacteria at 2 h: 77 ± 8% in WT (n = 9 in triplicate) vs. 129 ± 20% in F508del (n = 3 in triplicate), p = 0.04. Data are presented as mean ± SEM. Statistical significance from unpaired nonparametric Wilcoxon test. *p < 0.05.