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. 2019 Apr 24;9:6516. doi: 10.1038/s41598-019-42751-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LL-37 and hBD1 antimicrobial capacities against S. aureus CIP 76.25 at different pH. LL-37 and hBD1 antimicrobial peptides were incubated at 50 µg/mL for 2 h with 100,000 CFU/mL of S. aureus CIP 76.25 in culture medium at pH = 7,00; 7.40 and 7.70. Solutions were plated at the end of the experiments on Petri dishes to count the surviving bacteria. Surviving bacteria are expressed as % from the inoculum under different conditions. (A) LL-37 activity evaluated at: pH = 7.00 (3 different cultures in duplicate), 7.40 (2 different cultures in duplicate) and 7.70 (n = 3 in duplicate), p = 0.01. (B) hBD1 activity evaluated at: pH = 7.00, 7.40 and 7.70, p = 0.03. For all conditions, n = 2 in duplicate. Data are presented as mean ± SEM. Statistical significance from unpaired nonparametric Kruskal-Wallis test. *p < 0.05.