Table 1.
Concordance between qPCR and Fluidigm for the detection of common nasopharyngeal pathogens in PCV-vaccinated, HIV-uninfected children.
| Bacterial Target | qPCR | Fluidigm | P-value | Concordance (kappa) | Sensitivity (%) | Specificity (%) |
|---|---|---|---|---|---|---|
| S. pneumoniae | 244(72.8) | 246(73.4) | 0.16 | 0.98 | 98.8 | 100 |
| H. influenzae | 230(68.7) | 232(69.3) | 0.32 | 0.97 | 98.7 | 99 |
| M. catarrhalis | 189(56.4) | 192(57.3) | 0.08 | 0.98 | 98.4 | 100 |
| S. aureus | 57(17) | 55(16.4) | 0.16 | 0.98 | 100 | 99.3 |
| S. pyogenes | 11(3.3) | 12(3.6) | 0.32 | 0.96 | 91.7 | 100 |
| N. meningitidis | 3(0.9) | 5(1.5) | 0.16 | 0.75 | 91.7 | 100 |