Table 4.
Liver and kidney glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), total antioxidant activity (TAC), nitric oxide (NO) and thiobarbituric acid-reactive substances (TBARS) of male rats treated with aluminum oxide nanoparticles (Al2O3NPs), zinc oxide nanoparticles (ZnONPs) and their combination.
| Parameter | Experimental groups |
|||
|---|---|---|---|---|
| Control | Al2O3NPs | ZnONPs | Combination | |
| Liver | ||||
| GSH(μmole/gm tissue) | 5.4 ± 0.27a | 3.9 ± 0.07b | 3.8 ± 0.09b | 3.1 ± 0.07c |
| SOD (U/mg protein) | 53.1 ± 2.5a | 40.6 ± 2.4b | 42.6 ± 3.4b | 35.5 ± 1.4c |
| CAT (U/mg protein) | 66 ± 0.68a | 50 ± 0.77c | 53 ± 0.62b | 40 ± 0.42d |
| GPx (U/mg protein) | 25.9 ± 0.45a | 19.8 ± 0.39 b | 18.9 ± 0.44b | 15.8 ± 0.35 c |
| GST(μmole /hr/mg protein) | 1.77 ± 0.05a | 1.38 ± 0.05 b | 1.39 ± 0.05b | 1.06 ± 0.05c |
| TAC (μmole/gm tissue) | 2.5 ± 0.09a | 1.97 ± 0.05b | 1.9 ± 0.11b | 1.58 ± 0.05c |
| NO (μmole/gm tissue) | 0.47 ± 0.03c | 0.60 ± 0.03b | 0.63 ± 0.05b | 0.77 ± 0.04a |
| TBARS (nmole/gm tissue) | 45 ± 2.0c | 71 ± 2.2b | 75 ± 4.4b | 87 ± 3.2 a |
| Kidney | ||||
| GSH (μ mole/gm tissue) | 5.7 ± 0.14a | 4.5 ± 0.09b | 4.5 ± 0.12b | 3.9 ± 0.09c |
| SOD (U/mg protein) | 60.8 ± 1.1 a | 36.5 ± 1.1 c | 39.0 ± 1.0 b | 33.1 ± 0.8d |
| CAT (U/mg protein) | 53.9 ± 2.36a | 34.7 ± 0.79 b | 34.3 ± 1.02 b | 25.7 ± 1.10c |
| GPx (U/mg protein) | 84.1 ± 0.93a | 65.6 ± 1.13 b | 63.6 ± 1.16 b | 51.6 ± 1.86c |
| GST(μmole /hr/mg protein) | 1.16 ± 0.03a | 0.92 ± 0.02 b | 0.93 ± 0.02 b | 0.77 ± 0.02c |
| TAC (μmole/gm tissue) | 2.5 ± 0.06a | 1.8 ± 0.06b | 1.9 ± 0.08b | 1.5 ± 0.06c |
| NO (μmole/gm tissue) | 0.5 ± 0.02c | 0.6 ± 0.04b | 0.7 ± 0.03b | 0.8 ± 0.03a |
| TBARS (nmole/gm tissue) | 22.4 ± 1.53c | 68.2 ± 1.29 b | 70.4 ± 1.19 b | 80.8 ± 1.27a |
Data presented as Mean±SE. For each parameter, the groups with different superscript letters are significantly differ, p < 0.05.