Table 2.
Significantly regulated proteins (⇧ upregulation - ⇩ downregulation) under different conditions. Function based on UniProt database search. Protein names and symbol are according to Hugo Gene Nomenclature Committee – synonyms/previous names are italicised.
| Protein name (gene name) synonym | Function | Fold change in Pure-Mg | Fold change in Mg-10Gd | Fold change in Mg-2Ag | |
|---|---|---|---|---|---|
| Significantly regulated proteins in Mg-10Gd and Mg-2Ag (not in Pure-Mg). | Charged multivesicular body protein 4B (CHMP4B) Chromatin modifying protein 4B | negative regulation of cell death | / | ⇧ 2.29 ±0.199 |
⇧ 2.57 ±0.068 |
| Eukaryotic translation initiation factor 3 subunit C (EIF3C) | translation initiation factor activity | / | ⇩ 2.04 ±0.0.97 |
⇩ 2.34 ±0.056 |
|
| Thioredoxin reductase 1 (TXNRD1) | cell proliferation, response to reactive oxygen species, thioredoxin-disulfide reductase activity | / | ⇩ 2.14 ±0.02 |
⇩ 2.24 ±0.018 |
|
| Keratin 19 (KRT19) | cell differentiation, involved in embryonic placenta development, structural constituent of cytoskeleton | / | ⇩ 16.22 ±0.319 |
⇩17.78 ±0.068 |
|
| Asparagine-tRNA ligase (NARS) | negative regulation of apoptotic process, response to toxic substance | / | ⇩ 4.17 ±0.083 |
⇩ 5.13 ±0.069 |
|
| Fold change in Pure-Mg | Fold change in Mg-10Gd | Fold change in Mg-2Ag | |||
| Significantly regulated proteins in Mg-10Gd and Pure-Mg (not in Mg-2Ag) | Transmembrane p24 trafficking protein 7 (TMED7) Transmembrane emp24 domain-containing protein 7 | protein transport | ⇧ 3.39 ±0.077 |
⇧ 2.82±0.0056 | / |
| Sideroflexin 3 (SFXN3) | transporter activity | Not present in control | Not present in control | / | |
| IKBKB interacting protein (IKBIP) Inhibitor of nuclear factor kappa-B kinase-interacting protein | response to X-ray | ⇧ 2.09 ±0.036 |
⇧ 2.29 ±0.072 |
/ | |
| Pyrophosphatase (inorganic) 1 (PPA1) PP | magnesium ion binding; phosphate-containing compound metabolic process | ⇩ 2.45 ±0.022 |
⇩ 2.00 ±0.060 |
/ | |
| Bleomycin hydrolase (BLMH) | aminopeptidase activity, proteolysis, response to toxic substance | Not present in Pure-Mg | ⇩ 2.19 ±0.056 |
/ | |
| Four and a half LIM domains protein 1 (FHL1) LIM protein SLIMMER (SLIM1) | cell differentiation, positive regulation of potassium ion transport, zinc ion binding | ⇩ 3.31 ±0.194 |
⇩ 2.69 ±0.065 |
/ | |
| Nexilin F-actin binding protein (NEXN) NELIN, nexilin | regulation of cytoskeleton organisation | Not present in Pure-Mg | Not present in Mg-10Gd | / | |
| AHNAK nucleoprotein (AHNAK) neuroblast differentiation-associated protein, desmoyokin | regulation of voltage-gated calcium channel activity | ⇩ 2.57 ±0.098 |
⇩ 2.69 ±0.24 |
/ | |
| Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) lysyl hydroxlase 1, LH1 | oxidation-reduction process, procollagen-lysine 5-dioxygenase activity | ⇧ 2.09 ±0.020 |
⇧ 2.00 ±0.133 |
/ | |
| tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE) 14-3-3 protein epsilon | negative regulation of cysteine-type endopeptidase activity involved in apoptotic process, positive regulation of protein insertion into mitochondrial membrane, involved in apoptotic signalling pathway | ⇩ 2.09 ±0.117 |
⇩ 2.24 ±0.178 |
/ | |
| Prostaglandin-endoperoxide synthase 2 (PTGS2) prostaglandin G/H synthase 2, cyclooxygenase 2, COX2 | positive regulation of apoptotic process, angiogenesis, involved in sprouting angiogenesis, bone mineralisation | Not present in control | Not present in control | / | |
| tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) protein kinase C inhibitor protein 1 | positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signalling pathway | ⇩ 2.24 ±0.067 |
⇩ 2.75 ±0.078 |
/ | |
| ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1(ATP5F1) | ATP biosynthetic process, transporter activity | ⇧ 2.19 ±0.061 |
⇧ 2.63 ±0.132 |
/ | |
| Galectin 3 (LGALS3) lectin, galactoside-binding, soluble, 3 | epithelial cell differentiation, regulation of extrinsic apoptotic signalling pathway via death domain receptors, regulation of T cell apoptotic process, regulation of T cell proliferation | Not present in Pure-Mg | Not present in Mg-10Gd | / | |
| Plastin 3 (PLS3) T-plastin | auditory receptor cell differentiation, bone development, calcium ion binding | ⇩ 2.04 ±0.071 |
⇩ 2.09 ±0.068 |
/ | |
| Cytochrome c oxidase subunit 4 isoform 1 (COX4I1) cytochrome c oxidase subunit IV, COX4 | generation of precursor metabolites and energy, response to nutrient | ⇧ 2.19 ±0.066 |
⇧ 2.04 ±0.041 |
/ | |
| Calpain 1 (CAPN1) calpain 1, (mu/I) large subunit | extracellular matrix disassembly, positive regulation of cell proliferation, proteolysis | ⇩ 3.47 ±0.083 |
⇩ 3.16 ±0.061 |
/ | |
| Lecithin-cholesterol acyltransferase (LCAT) phosphatidylcholine-sterol acyltransferase | lipoprotein biosynthetic process, response to copper ion | Not present in control | Not present in control | / | |
| H2A histone family, member (H2AFY) Core histone macro-H2A.1, MACROH2A1 | SH3/SH2 adaptor activity | ⇧ 2.04 ±0.099 |
⇧ 2.29 ±0.108 |
/ | |
| Actinin alpha 4 (ACTN4) | BAT3 complex binding, positive regulation of ER-associated ubiquitin-dependent protein catabolic process | ⇩ 2.09 ±0.035 |
⇩ 2.19 ±0.027 |
/ | |
| Orosomucoid 1 (ORM1) alpha-1-acid glycoprotein 1, OMD, ORM | metal ion binding, SMAD protein signal transduction, transport | Not present in Pure-Mg | Not present in Mg-10Gd | / | |
| Alpha fetoprotein (HPAFP) | oxygen transporter activity, heme binding | ⇩ 2.51 ±0.0137 |
⇩ 2.88 ±0.202 |
/ | |
| Fold change in Pure-Mg | Fold change in Mg-10Gd | Fold change in Mg-2Ag | |||
| Significantly regulated proteins in Mg-2Ag and Pure-Mg (not in Mg-10Gd) | REX2, RNA exonuclease 2 homolog (S. cerevisiae) (REXO2), Oligoribonuclease, mitochondrial precursor | 3′-5′ exonuclease activity, focal adhesion, nucleotide metabolic process | Not present in control | / | Not present in control |
| LIM domain and actin-binding protein 1 (LIMA1), Epithelial protein lost in neoplasm, EPLIN, FLJ38853 | focal adhesion, negative regulation of actin filament depolymerisation, stress fiber | ⇩ 4.07 ±0.036 |
/ | ⇩ 2.51 ±0.112 |
|
| Reticulon-4 (RTN4), ASY, Foocen, KIAA0886, My043, Nbla00271 | negative regulation of cell growth, of apoptotic process, regulation of cell migration | ⇩ 2.19 ±0.160 |
/ | ⇩ 2.14 ±0.14 |
|
| Histone cluster 1, H2bl (HIST1H2BL) Histone H2B type 1-L, H2BFC, Histone H2B.c | nucleosome assembly | ⇧ 2.24 ±0.044 |
/ | ⇧ 2.75 ±0.035 |
|
| Kinectin (KTN1), CG1, CG-1 antigen, kinesin receptor | microtubule-based movemen | Not present in Pure-Mg | / | Not present in Mg2Ag | |
| non-POU domain containing, octamer-binding (NONO), NonO protein, Non-POU domain-containing octamer-binding protein | DNA recombination, DNA repair, negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [ | ⇧ 2.45 ±0.097 |
/ | ⇧ 2.24 ±0.040 |
|
| LIM and SH3 domain protein 1 (LASP1), Metastatic lymph node gene 50 protein,Lasp-1 | focal adhesion;ion transmembrane transporter activity, ion transport, zinc ion binding | ⇩ 4.07 ±0.089 |
/ | ⇩ 2.88 ±0.23 |
|
| Major vault protein (MVP), LRP, Lung resistance-related protein, Major vault protein | ERBB signaling pathway, protein transport | ⇧ 2.29 ±0.115 |
/ | ⇧ 2.04 ±0.006 |
|
| Caldesmon 1 (CALD1), CDM, L-caldesmon, Non-muscle caldesmon | movement of cell or subcellular component, muscle contraction | ⇩ 3.09 ±0.076 |
/ | ⇩ 2.04 ±0.090 |
|
| Nucleobindin 1 (NUCB1), CALNUC, DKFZp686A15286 | regulation of protein targeting | ⇧ 2.34 ±0.108 |
/ | ⇧ 2.40 ±0.008 |
|
| heat shock 10 kDa protein 1 (chaperonin 10), (HSPE1), 10 kDa heat shock protein, 10 kDa chaperonin, CPN10 | activation of cysteine-type endopeptidase activity involved in apoptotic process, osteoblast differentiation | ⇧ 2.24 ±0.107 |
/ | ⇧ 2.24 ±0.029 | |
| Branched-chain-amino-acid aminotransferase 1, cytosolic (BCAT1), BCAT(c), BCATC, BCT1, Branched-chain-amino-acid aminotransferase, cytosolic | aspartate biosynthetic process, cell proliferation | ⇩ 2.24 ±0.034 |
/ | ⇩ 2.14 ±0.103 |
|
| Tu translation elongation factor, mitochondrial (TUFM), Elongation factor Tu, mitochondrial, P43, COXPD4 | GTP binding, GTPase activity, mitochondrial translational elongation [ | ⇩ 2.29 ±0.041 |
/ | ⇩ 2.29 ±0.105 |
|
| Serum amyloid A4, constitutive (SAA4), Serum amyloid A-4 protein, CSAA, C-SAA | acute-phase response, cell chemotaxis, chemoattractant activity | Not present in control | / | Not present in control | |
| Phosphatidylethanolamine-binding protein 1 (PEBP1), HCNP, HCNPpp, Neuropolypeptide h3, PBP | ATP binding, serine-type endopeptidase inhibitor activity | ⇩ 3.89 ±0.110 |
/ | ⇩ 2.88 ±0.117 |
|
| Filamin-A, akoga (FLNA), ABP-280, ABPX, Actin-binding protein 280, Alpha-filamin | cell junction assembly, focal adhesion, negative regulation of apoptotic process, wound healing | ⇩ 2.14 ±0.062 |
/ | ⇩ 2.09 ±0.020 |
|
| Spermidine synthase (SRM), PAPT, Putrescine aminopropyltransferase, SPDSY | polyamine metabolic process, protein homodimerization activity, spermidine biosynthetic process | ⇩ 2.29 ±0.152 |
/ | ⇩ 2.40 ±0.067 |
|
| Ezrin (EZR), CVIL, CVL, Cytovillin, DKFZp762H157, Ezrin | focal adhesion, regulation of cell shape | ⇩2.19 ±0.116 |
/ | ⇩ 2.29 ±0.017 |
|
| Heat shock 60 kDa protein 1 (chaperonin) (HSP60), 60 kDa heat shock protein,Chaperonin 60, Chaperonin 60, CPN60, GROE | negative regulation of apoptotic process, positive regulation of interleukin-6,10,12 | ⇧ 2.14 ±0.099 |
/ | ⇧ 2.00 ±0.045 |
|
| l-lactate dehydrogenase B (LDHB), l-lactate dehydrogenase B chain, LHD heart subunit, LHD-B | l-lactate dehydrogenase activity, pyruvate metabolic process | ⇩ 2.04 ±0.034 |
/ | ⇩ 2.09 ±0.049 |
|
| Apolipoprotein A-II (APOA2), apoAII, ApoA-II, Apo-AII, Apolipoprotein A2 | acute inflammatory response, protein oxidation | Not present in control | / | Not present in control | |
| Crystallin alpha B (CRYAB), Alpha-crystallin B chain, Alpha (B)-crystalline, HspB5, HSPB5 | negative regulation of extrinsic apoptotic signaling pathway, positive regulation of cell aging, positive regulation of osteoblast differentiation, negative regulation of adipose tissue development, | ⇧ 3.02 ±0.084 |
/ | ⇧ 2.45 ±0.048 |
|
| Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), A1A, A1AT, Alpha-1-antiproteinase, alpha-1-antitrypsin | acute-phase response, inflammatory response, serine-type endopeptidase inhibitor activity | Not present in control | / | Not present in control | |
| Serpin peptidase inhibitor, clade C (antithrombin), member 1 (SERPINC1) Antithrombin-III, AT3, ATIII, Serpin C1 | acute-phase response, serine-type endopeptidase inhibitor activity | Not present in control | / | Not present in control | |
| Haptoglobin-related protein (HPR), A-259H10.2, Haptoglobin-related protein, HP | extracellular matrix disassembly, negative regulation of cell proliferation, negative regulation of cell-cell adhesion mediated by cadherin, positive regulation of fibrinolysis, serine-type endopeptidase activity | Not present in control | / | Not present in control | |
| Glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), Aspartate aminotransferase, mitochondrial, FABP-1, FABPpm | canonical glycolysis,epithelial cell differentiation | ⇧ 2.63 ±0.088 |
/ | ⇧ 2.51 ±0.48 |
|
| Glutaredoxin 3 (GLRX3), Glutaredoxin-3, GRX3, GRX4, PICOT, PKC-interacting cousin of thioredoxin | osteoblast differentiation, regulation of apoptotic process | Not present in P-Mg | / | Not present in Mg-2Ag | |
| Programmed cell death protein 5 (PDCD5), Protein TFAR19, TF-1 cell apoptosis-related protein 19, TFAR19 | chloride transmembrane transport, lipid transport | ⇩ 3.63 ±0.067 |
/ | ⇩ 2.63 ±0.196 |
|
| Importin5 (IPO5), Imp5, Importin-5, Importin subunit beta-3 | positive regulation of apoptotic process, positive regulation of intrinsic apoptotic signaling pathway | ⇩2.14 ±0.039 |
/ | ⇩ 2.19 ±0.008 |