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. 2019 Apr 11;15:28–38. doi: 10.1016/j.isci.2019.04.013

Figure 1.

Figure 1

Design of GRAPHIC and Its Signal Pattern

(A) Diagram of GRAPHIC molecular structures. GRAPHIC molecules consist of signal peptide (SP), split sfGFP fragment, leucine zipper domains (LZA or LZB), and GPI anchor domain. To identify and estimate probe expression, H2B-mCherry and H2B-Azurite were co-expressed with NT-probe and CT-probe, respectively.

(B) GFP molecule consists of 11 β-sheets and fluorophore domain (FL). sfGFP split site for GRAPHIC is between seventh and eighth β-sheet (7/8). Other intercellular probe systems utilize 10/11 split site.

(C) Diagram of GRAPHIC labeling intercellular contact. GFP molecules are reconstituted by intercellular interaction of a set of probe molecules at cell-cell contact site.