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. 2019 Apr 11;15:28–38. doi: 10.1016/j.isci.2019.04.013

Figure 2.

Figure 2

Comparison of GRAPHIC with Other Probes

(A) GRAPHIC showed higher signal intensity in LLCPK1 epithelial cell culture. Modification of GFP split site (10/11 to 7/8) in mGRASP system increased its signal intensity. All three culture and image acquisition conditions are same.

(B) Quantification and comparison of GRAPHIC (n = 57) and modified mGRASP (7/8) (n = 66) signal intensity. GFP signals were normalized with co-expressed nuclei label intensities of contacted cells. GFP-NT fragment (post-mGRASP and n-GRAPHIC)-expressing cells are red nuclei (H2B-mCherry, attached with 2A peptide) and GFP-CT fragment (pre-mGRASP and c-GRAPHIC)-expressing cells are blue nuclei (H2B-Azurite, attached with 2A peptide). ∗∗∗p = 1.09 × 10−24; Student's unpaired t test. Scale bars, 20 μm.