Figure 2.

Accuracy of minor variant frequencies. Panel (A) shows the consistency of minor variant frequencies across two replicate extraction, RT-PCR, and sequencing of two samples. Minor variation was relatively consistently recovered down to a frequency of about 1 per cent. Panel (B) shows the distribution of the frequencies of non-consensus calls across all sites covered in excess of 2,000-fold. At the majority of sites, no variation above 0.001 is observed. Note that this variation includes within sample variation, RT-PCR errors, and sequencing errors.