Fig. 4.

Binding of disulfide HMGB1 to CRM1. (A) IP of Myc–tagged HMGB1(WT), HMGB1(C23S) or HMGB1(C45S) with CRM1 after 50 μM H₂O₂ or 0.5 mU/mL GOX for 2 h stimulation in HEK293T. (B) HEK293T cells expressing Myc–tagged WT, C23S, or C45S forms of HMGB1 were incubated in the absence or presence of 50 μM H₂O₂ or GOX (5 mU/mL) for 2 h and then subjected to a PLA assay with antibodies to Myc and to CRM1. Scale bars, 10 μm.