Fig. 5.

Deficiency of PrxI or PrxII attenuates HMGB1 secretion in BMDMs and vice versa. (A-D) BMDMs derived from WT, PrxI-KO (PrxI^–/–), or PrxII-KO (PrxII^–/–) mice were incubated in the absence or presence of 100 ng/mL LPS, 250 nM PMA, 50 ng/mL TSA, or 50 ng/mL TNFα for 24 h, after which culture supernatants were subjected to IB analysis. Means ± SEM (n = 3). (E, F) PrxII KO MEF cells were transfected with PrxII plasmid and incubated with the indicated concentrations of LPS (ng/mL) (E) or GOX (μU/ml) (F) for 24 h, after which culture supernatants were subjected to IB analysis. WT MEF cells were used for positive control. Means ± SEM (n = 3). *P < 0.05, **P < 0.01 versus corresponding value for PrxII KO cells, t-test.