Figure 2.

Genotoxic Stress Induces the Relocation of P-TEFb and RBM7 from 7SK snRNP to Pol II

(A and B) CoIP of F-RBM7 (A) and F-LARP7 (B) with 7SK snRNP from WCE of HEK293 cells. Conditions with (+) and without (−) 4-NQO are shown.

(C and D) CoIP of F-CDK9 (C) and F-RBM7 (D) with the indicated proteins from WCE of HEK293 cells. Conditions with (+) and without (−) 4-NQO are shown.

(E) Cartoon depicting the V-PAC assay. P-TEFb-releasing agents induce the transfer of the inactive YC-P-TEFb (inactive CDK9 in red) to the substrate YN-CTD chimera (active CDK9 in green), yielding YFP fluorescence.

(F) V-PAC assay in HeLa cells expressing YC-P-TEFb and YN-CTD chimera. Left: representative YFP fluorescence (YFP) and phase contrast (cells) images of cells are shown. Conditions with (in hours) and without (−) 4-NQO are shown. Right: quantification of YFP-positive cells that were treated as indicated.

See also Figure S2.