Table 2.
Primary antibodies | Source | Catalog # | Dilution |
---|---|---|---|
NGF Pro-NGF |
Alomon Labs (Jerusalem, Israel) | AN-240 ANT-005 |
1:500 1:500 |
GAPDH | Cell Signaling (Danvers, MA, USA) | 2118L | 1:1000 |
polyclonal anti-p75NTR | Bruce Carter | 1:3000 | |
TrkA pTrkA |
Abcam (Cambridge, MA, USA) | Ab 76291 Ab-1445 (Y490) |
1:1000 1:500 |
VEGF | Millipore (Billerica, MA, USA) | ABS82 | 1:1000 |
Cleaved-Caspase-1 | Enzo Life sciences Farmingdale, NY, USA |
ALX-210–804-C100 | 1:500 |
Cleaved-IL-1b | Abcam, Cambridge, MA, USA | ab82558 | 1:500 |
TNF-α | Novus Biologicals, Littleton, CO, | NBP1–19532 | 1:500 |
Actin | Sigma | A5060 | 1:1000 |