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. 2019 Apr 25;20:316. doi: 10.1186/s12864-019-5670-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Gene expression profiles for pituitary hormones in HG and LG chickens. Levels of mRNA for POMC (a), TSHβ (b), GH (c), PRL (d), FSHβ (e), LHβ (f), and CGA (g) were determined on post-hatch weeks 1, 3, 5, and 7 and normalized to levels of GAPDH mRNA. Values (mean + SEM; n = 4 birds per line) are expressed relative to the age and line with the highest expression level (set to 100% prior to log₂-transformation for statistical analysis). The line-by-age interaction was not significant for any hormone (P ≤ 0.05). Significant main effects of line and/or age (P ≤ 0.05) are indicated at the top of each graph, when present