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. 2019 Apr 25;20:316. doi: 10.1186/s12864-019-5670-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Self-organizing maps (SOMs) analysis of DEGs in the anterior pituitary of HG and LG chickens. Genes with a significant line-by-age interaction or a significant main effect of line (P ≤ 0.05; n = 4 birds per line) as determined by two-way ANOVA and a difference in expression of at least 1.6-fold between HG and LG lines (291 total) were organized into 16 clusters (c0-c15) in a 4 × 4 configuration. Data were organized into clusters as mean log₂-ratio of HG – mean log₂-ratio of LG. The number of genes contained within each cluster is indicated in parentheses at the top center of each cluster. Examples of important genes upregulated in LG chickens (green) or HG chickens (red) are identified in c4 (GH), c14 (TSHβ and LHβ) and c15 (FSHβ)