Fig. 2.

EHD1-induced angiogenic effects depend on VEGFA expression. a mRNA expression of VEGFA. b Correlation between EHD1 and VEGFA expression in the TCGA database (R = 0.14, p < 0.0001). c Western blot analysis of VEGFA expression in A549 and NCI-H1650 cells after EHD1 knockdown. d qRT-PCR analysis of VEGFA mRNA levels in A549 cells and NCI-H1650 cells. e A549 and NCI-H1650 cells were incubated overnight with serum-free 1640 medium, and their CMs were used to detect the level of VEGFA secretion; the lanes in the left and right show Ctrl and Sh, respectively. f CMs were added to the lower chamber, and HUVECs were seeded on the upper chamber. After 24 h of incubation, HUVEC migration was assessed by counting the cells on the lower surface of the membrane. Scale bar, 100 μm. g HUVECs were incubated in 48-well plates with CMs from A549 and NCI-H1650 cells, and their tube formation abilities were evaluated based on the number of tubes per field