Fig. 6.

Targeting of VEGFA represses EHD1-induced tumor growth and angiogenesis in vivo. a-b The expression of EHD1, β2AR and VEGFA in freshly frozen xenograft tumor tissue was analyzed by Western blot. c-g A549 luciferase cells stably expressing EHD1 ShRNA/R-Ctrl or EHD1 ShRNA/R-apatinib were transplanted into the alar skin of nude mice. Images of the resulting tumors in the mice on days 7 and 28 are shown (c). A photograph of the tumors on day 28 is shown (left), and the tumor growth curves of the mice on days 7 to 28 are presented (right) (d). The tumor weights were measured, and the data are presented as the means±SDs (n = 5, each) (e). Representative images of IHC staining for VEGFA and CD31 in xenograft tumor tissues are presented (f). Representative immunofluorescent staining of CD31 is shown (g). h Model of EHD1-induced β2AR/VEGFA expression and angiogenesis in NSCLC