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. 2019 Apr 25;15:119. doi: 10.1186/s12917-019-1861-5

Table 1.

Primers used for the detection of capsular types and virulence-associated genes in Pasteurella multocida isolates

Gene function Target gene Description Sequence (5′ – 3′) size (bp) Reference
Capsule serotypes kmt1 Identification of all P. multocida isolates ATCCGCTATTTACCCAGTGG
GCTGTAAACGAACTCGCCAC
460 [19]
hyaD-hyaC Serogroup A cap gene TGCCAAAATCGCAGTGAG
TTGCCATCATTGTCAGTG
1044 [20]
bcbD Serogroup B cap gene CATTTATCCAAGCTCCACC
GCCCGAGAGTTTCAATCC
760 [20]
dcbF Serogroup D cap gene TTACAAAAGAAAGACTAGGAGCCC
CATCTACCCACTCAACCATATCAG
657 [20]
ecbJ Serogroup E cap gene TCCGCAGAAAATTATTGACTC
GCTTGCTGCTTGATTTTGTC
511 [20]
fcbD Serogroup F cap gene AATCGGAGAACGCAGAAATCAG
TTCCGCCGTCAATTACTCTG
851 [20]
Outer membrane and porin proteins oma87 Outer membrane protein 87 ATGAAAAAACTTTTAATTGCGAGC
TGACTTGCGCAGTTGCATAAC
948 [17]
ompH Outer membrane protein H CGCGTATGAAGGTTTAGGT
TTTAGATTGTGCGTAGTCAAC
438 [17]
plpB Outer membrane protein TTTGGTGGTGCGTATGTCTTCT
AGTCACTTTAGATTGTGCGTAG
282 [18]
psl Porin protein TCTGGATCCATGAAAAAACTAACTAAAGTA
AAGGATCCTTAGTATGCTAACACAGGACGACG
470 [17]
Adhesins fimA Fimbriae CCATCGGATCTAAACGACCTA
AGTATTAGTTCCTGCGGGTG
866 [18]
pfhA Filamentous haemagglutinin AGCTGATCAAGTGGTGAAC
TGGTACATTGGTGAATGCTG
275 [21]
ptfA Fimbriae TGTGGAATTCAGCATTTTAGTGTGTC
TCATGAATTCTTATGCGCAAAATCCTGCTGG
488 [17]
hsf-1 Autotransporter adhesion TTGAGTCGGCTGTAGAGTTCG
ACTCTTTAGCAGTGGGGACAACCTC
654 [18]
hsf-2 Autotransporter adhesion ACCGCAACCATGCTCTTAC
TGACTGACATCGGCGGTAC
433 [18]
Superoxide dismutases sodA Superoxide dismutases TACCAGAATTAGGCTACGC
GAAACGGGTTGCTGCCGCT
361 [17]
sodC Superoxide dismutases AGTTAGTAGCGGGGTTGGCA
TGGTGCTGGGTGATCATCATG
235 [17]
Iron acquisition related factor exbB Iron regulated and acquisition factors TTGGCTTGTGATTGAACGC
TGCAGGAATGGCGACTAAA
283 [18]
exbBD-tonB Iron acquisition related factors GGTGGTGATATTGATGCGGC
GCATCATGCGTGCACGGTT
1144 [17]
fur Iron regulated and acquisition factors GTTTACCGTGTATTAGACCA
CATTACTACATTTGCCATAC
244 [18]
tbpA Iron acquisition related factor TGGTTGGAAACGGTAAAGC
TAACGTGTACGGAAAAGCC
728 [21]
hgbA Haemoglobin binding protein TGGCGGATAGTCATCAAG
CCAAAGAACCACTACCCA
419 [17]
hgbB Haemoglobin binding protein TCATTGAGTACGGCTTGAC
CTTACGTCAGTAACACTCG
499 [21]
Neuraminidases nanB Neuraminidases GTCCTATAAAGTGACGCCGA
ACAGCAAAGGAAGACTGTCC
554 [17]
nanH Neuraminidases GAATATTTGGGCGGCAACA
TTCTCGCCCTGTCATCACT
360 [17]
Hyaluronidase pmHAS Hyaluronidase TCAATGTTTGCGATAGTCCGTTAG
TGGCGAATGATCGGTGATAGA
430 [18]
Toxin toxA Dermonecrotic toxin TCTTAGATGAGCGACAAGG
GAATGCCACACCTCTATAG
846 [21]