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. 2019 Apr 25;20:80. doi: 10.1186/s13059-019-1678-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — sgRNA design and expression profiling of CRISPR/Cas9 transgenics. a Low off-target sgRNAs targeting the DNA A of the African cassava mosaic virus and in silico predicted efficiency score (max. efficiency= 1). b In vitro cleavage assay for testing the effectiveness of six different sgRNAs against the viral template. c, d Reverse transcription-quantitative PCR (RT-qPCR) analysis of Cas9 and sgRNA transgene expression respectively. Three independent plants per line were tested