Fig. 6.

RBP-Jκ knockdown regulates PTEN and EMT-associated genes expression. a Western blot analyses showing the protein level of PTEN, FAK, p-FAK, RBP-Jκ and EMT-related genes (N-cadherin, E-cadherin, Snail-1, MMP-9) in NC (sh-NC) or RBP-Jκ-knockdown (RBP-Jκ shRNA) SCC-9 and CAL-27 cells. GAPDH serves as internal control. b Gray level analysis of the protein level of Numb, Notch1, RBP and EMT-related genes (E-cadherin, N-cadherin, Snail-1, MMP-9) in mock or Numb-overexpressed SCC-9 and CAL-27 cells after western blot. The mean ± SD represents the average levels from 3 replicates. *p < 0.05, **p < 0.01