Fig. 3.
Relative gene expression levels of HNSCC pathogenesis-specific genes in clinical tissues. a Graphical representation of qRT-PCR results (using SYBR Green chemistry). b Graphical representation of qRT-PCR results (using TaqMan chemistry). The graphs show the relative fold change values/relative gene expression of various genes involved in HNSCC pathogenesis. Histograms represent the levels of up/down-regulation of a gene as compared to control samples. Relative gene expression was obtained after normalization with endogenous human GAPDH and determination of the difference in threshold cycle (Ct) between tumor and normal tissues was performed using the 2-ΔΔCt method. All the qRT-PCR assays were performed in triplicate. The results are expressed as means±standard errors of the mean. *, p < 0.05; **, p < 0.01; ***, p < 0.001 and ****, p < 0.0001 represent the statistical significance compared with control