Fig. 6.

Effects of GA and ABA on recovering the advanced-germination phenotype of the BnPHT1;4 overexpression transgenic Brassica napus seeds. (a and b) effects of GA and PAC (paclobutrazol, a GA biosynthesis inhibitor) on seed germination. Seeds were treated with 0.1 mM GA or 10 mM PAC. One representative image per genotype (1.5 days after sowing) is shown in a. Quantitative analysis of germination rates are shown in b. The BnPHT1;4 transgenic seeds (S10 and S11) germinated more rapidly than those of the controls (WT and CK) under normal conditions (Mock). PAC delayed germination of the BnPHT1;4 transgenic seeds, while GA promoted seed germination of the controls. (c and d) effects of ABA on seed germination. Seeds were treated with 5, 50 and 100 μM ABA. One representative image per genotype (1.5 days after sowing) is shown in c. Quantitative analysis of germination rates are shown in d. ABA partially recovered the advanced-germination phenotype of the BnPHT1;4 transgenic seeds. The data are presented as means ± SD (n = 5). WT, wild type; CK, transgenic null line; S10 and S11, independent BnPHT1;4 overexpression transgenic lines