Figure 2.

RBM48 Is Missing in Some Eukaryotic Clades.

(A) Species tree including significant eukaryotic model organisms. Gray boxes indicate lineages that have lost U12-type introns. Drosophila melanogaster (fly) encodes a hypothetical gene with an atypical RBM48 RRM-like domain.

(B) Maximum likelihood tree of the RBM48_RRM domain. Bootstrap values ≥50 are reported in the corresponding nodes. The scale bar indicates the number of substitutions per site. Protein schematics show the RBM48 domain in blue. Protein sequences are from Zea mays (maize), Arabidopsis thaliana (Arabidopsis), Amborella trichopoda (Amborella), Ginkgo biloba (Ginkgo), Physcomitrella patens (Moss), Cyanophora paradoxa (Glaucophyta), Homo sapiens (Human), Mus musculus (Mouse), Gallus gallus (Chicken), Xenopus tropicalis (Frog), Danio rerio (Zebrafish), Rhizophagus irregularis DAOM 181602 (Fungi), and Phytophthora sojae (Oomycetes).

(C) Multiple sequence alignment of representative RBM48 domains. Asterisks indicate completely conserved sites. Ribonucleoprotein domains (RNP1, RNP2) of the predicted β1- and β3-seets in the RRM are indicated.