Figure 4.

U12-Type Intron Retention in rbm48 Mutants.

(A) RNA-seq read depth of GRMZM2G131321, GRMZM2G097568, and GRMZM2G052178, homologs of human SPCS2, TRAPPC2, and BYSL, respectively. Each panel is the summed read depth of four biological replicate libraries of mutant and normal sibling (WT, wild type) endosperm tissues for rbm48-umu1 and rbm48-umu2 segregating ears. Red arrow and brace symbol indicate the U12-type intron with increased read depth in rbm48 mutants.

(B) RT-PCR of normal (N) and rbm48 mutant (m) RNA from endosperm and seedling shoot tissues. Schematics show amplified products with PCR primers indicated by arrows.