BiFC Interactions of RBM48 with RGH3, U2AF1, and U2AF2.
BiFC assays showing transient co-expression in N. benthamiana leaves of fusion proteins with N-terminal EYFP (nEYFP) or C-terminal EYFP (cEYFP). Yellow signal indicates reconstituted EYFP. DAPI staining of DNA marks nuclei in blue.
(A) Co-expression of four combinations of RBM48 and RGH3 N- and C-terminal fusion proteins as well as the RGH3ΔUHM in-frame domain deletion.
(B) Co-expression of RBM48 and U2AF1 fusions.
(C) Co-expression of RBM48 and U2AF2 fusions.