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. 2018 Dec 11;31(3):663–686. doi: 10.1105/tpc.18.00437

Figure 2.

Figure 2.

ABRE Motifs Mediate H3K9ac Association and the Regulation of PtrNAC Genes.

(A) RT-qPCR detection of PtrNAC005, PtrNAC006, PtrNAC007, PtrNAC118, and PtrNAC120 in wild-type P. trichocarpa plants without (ND) or with drought treatment for 5 d (D5) and 7 d (D7). Error bars indicate 1 se of three biological replicates from independent pools of P. trichocarpa SDX tissues. Asterisks indicate significant differences between control (ND) and drought-treated (D5 and D7) samples for each gene (**, P < 0.01, Student’s t test).

(B) Schematic diagram of ABRE motifs in five PtrNAC gene promoters.

(C) ChIP-qPCR detection of H3K9ac in ABRE motif regions of PtrNAC promoters in wild-type P. trichocarpa plants without (ND) or with drought treatment for 5 d (D5) and 7 d (D7). Numbers indicate ABRE motif sites in each gene. ChIP assays were performed using antibodies against H3K9ac, and the precipitated DNA was quantified by qPCR. Enrichment values represent the relative fold change from ND, and error bars indicate 1 se of three biological replicates from independent pools of P. trichocarpa SDX tissues. Asterisks indicate significant differences between control (ND) and drought-treated (D5 and D7) samples for each fragment containing the ABRE motif (*, P < 0.05 and **, P < 0.01, Student’s t test).