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. 2019 Feb 13;31(3):627–644. doi: 10.1105/tpc.18.00854

Figure 1.

Figure 1.

Structural Polysaccharide Transport and Deposition, and Our Hybrid Methodology for Vesicle Glycomic Analysis.

(A) Schematic representation of structural polysaccharide synthesis, transport, and deposition.

The structural polysaccharides XyG and pectin are synthesized in the Golgi and transported via trans-Golgi–derived vesicles to the apoplast. The type of vesicles carrying specific polysaccharide cargo to the cell wall is unknown.

(B) Schematic representation of vesicle isolation and glycome identification. Plant extracts derived from liquid-grown plantlets are sucrose (Suc) fractionated. The enriched vesicles are isolated from the Golgi/trans-Golgi network–enriched Suc fractions with the aid of an Ab against a target protein. Green ellipsoids represent the SYP61 bait protein.

(C) Vesicle cargo release and glycome analysis. Vesicle cargo is released by sonication for glycome analysis. An ELISA-based method of glycome detection is used, and the resulting data are summarized in a heatmap for analysis. EE, early endosome; mAb, glycan-directed mAb; SV, secretory vesicle; TGN, trans-Golgi network.