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. 2019 Apr 24;11:1179543319840323. doi: 10.1177/1179543319840323

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Bacterial growth and induction of dsRNA production by IPTG. (A) Various concentrations of IPTG were treated at the mid-exponential phase (arrow). (B) GFP-dsRNA extracted from different IPTG treatments were run by 1.2% agarose gel electrophoresis. M indicates TrackIt 1Kb Plus DNA Ladder (Invitrogen). (C) Digestion of dsRNA by various nucleases. The purified bacterial GFP-dsRNA (Lane 1) was degraded by RNase III (Lane 2), but not by DNase I (Lane 3) or RNase A (Lane 4). dsRNA indicates double-stranded RNA; GFP, green fluorescent protein; IPTG, isopropyl β-d-1-thiogalactopyranoside; M, GeneRuler 1 kb DNA Ladder (Thermo Scientific).