Fig. 2.

Niemann-Pick C1 Like 1 (NPC1L1) protein is degraded by proteasomal pathway. A: stably transfected human intestinal HuTu-80 cells were treated with proteasome inhibitors, lactacystin (10 μM) or MG-132 (10 μM), for 24 h. Cells were then harvested, and total protein was isolated. NPC1L1 protein expression was detected by Western blotting using anti-hemagglutinin (HA) antibody. GAPDH was used as a loading control. B: densitometric analysis showed a significant increase in NPC1L1 protein expression in both the MG-132- and lactacystin-treated groups compared with control. Data are expressed as percent of control and presented as means ± SE from 3 different experiments. ****P < 0.0001 compared with control.