Fig. 5.

Inhibition of mitogen-activated protein kinase (MAPK)-ERK signaling decreased Niemann-Pick C1 Like 1 (NPC1L1) protein expression. A: stably transfected HuTu-80 cells were treated with MEK inhibitor PD-98059 (15 μM) or vehicle for 24 h, and cell lysates were analyzed for NPC1L1 protein expression by Western blot analysis. B: densitometric analysis showed a decrease in NPC1L1 protein expression in response to PD-98059. Data are presented as means ± SE from 3 different experiments. *P < 0.01 compared with control.