Fig. 7.

Effect of blocking proteasomal or lysosomal degradation pathways or mitogen-activated protein kinase (MAPK) inhibition on the cell surface levels of Niemann-Pick C1 Like 1 (NPC1L1). Stably transfected HuTu-80 cells were treated with MG-132, bafilomycin A1, and PD-98059 for 24 h, and surface biotinylation was performed. Biotinylated proteins were separated on SDS-polyacrylamide gel, and the blots were probed with anti-hemagglutinin (HA) and GAPDH antibodies. Representative blot of 3 separate experiments is shown.