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. 2019 Mar 13;316(4):C545–C558. doi: 10.1152/ajpcell.00023.2019

Fig. 5.

Fig. 5.

Disruption of NKCC1 COOH-terminal domain targets the cotransporter to the lysosomes. A–J: MDCK-WT and MDCK transfected with NKCC1 COOH-terminal mutants were cultured in MatTek Glass Bottom Microwell dishes. Cells were stained with PureBlu Hoechst 33342 and Lyso-Tracker Red and imaged live. WT, wild-type; 240, Δ46aa; 239, Δ92aa; DFX, Δ187aa, 238 Δ291aa; 237, Δ354aa; 236 Δ417aa; 241, LL1031-1032AA; 242, LL1192-1193AA; 244, LL1031-1032AA + LL1192-1193AA. Bars = 10 μm. GFP, green fluorescent protein; Lyso, lysosomes; MDCK, Madin-Darby canine kidney; NKCC1, Na+-K+-2Cl− cotransporter-1. Data are representative of 3 individual experiments where 5 live images per experiment were taken (Total, n = 15).