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. 2019 Mar 13;316(4):C545–C558. doi: 10.1152/ajpcell.00023.2019

Fig. 7.

Fig. 7.

NKCC1 DDxxxxLL1193–1194 dileucine motif is required to maintain the cotransporter in the basolateral membrane. MDCK cells were polarized on MatTek dishes for 3 days then transiently transfected with EGFP-NKCC1-WT (A), EGFP-NKCC1–240 (B), EGFP-NKCC1–239 (C), EGFP-NKCC1-DFX (D), EGFP-NKCC1–238 (E), EGFP-NKCC1–237 (F), EGFP-NKCC1–236 (G), EGFP-NKCC1–241 (H), EGFP-NKCC1–242 (I), and EGFP-NKCC1–244 (J). Cells were immunostained with anti-GFP and anti-ZO-1 antibody. WT, wild-type; 240, Δ46aa; 239, Δ92aa; DFX, Δ187aa, 238 Δ291aa; 237, Δ354aa; 236 Δ417aa; 241, LL1031-1032AA; 242, LL1192-1193AA; 244, LL1031-1032AA + LL1192-1193AA. Bars = 20 μm. GFP, green fluorescent protein; MDCK, Madin-Darby canine kidney; NKCC1, Na+-K+-2Cl− cotransporter-1. Data are representative of 3 individual experiments where 5 live images per experiment were taken (Total, n = 15).