Figure 4.

Incorporation of additional thioamide residues into the 4E-BP1 sequence does not significantly improve the signal-to-background ratio. A) Peptide sequences of thioamide-containing peptides (circled in orange). B) Fluorescence signals (λ_ex =494, λ_em =520 nm) of 10 μL of 10 nM FITC-4E-BP1 peptides in assay buffer in the absence or in the presence of 50 % TFE. Signal-to-background ratios were determined by dividing the fluorescence signal of each peptide in 50 % TFE by its fluorescence signal in assay buffer alone.