Figure 3.

Native agarose gels of P22 wtSP particles incubated with varying concentrations of SDS. (A) Increasing the SDS concentrations from 7 to 28 mM causes the capsid to disassemble as indicated by the bands containing species with higher electrophoretic mobility compared to the control bands (PC, 65 °C, and 75 °C) (Top) In Tris buffer. (Bottom) In phosphate buffer. (B) Comparison of samples exposed to standard protein denaturing conditions (420 mM SDS, 100 °C) to the 28 mM SDS expansion conditions. The migration of the bands is the same in all cases, suggesting capsid disassembly upon exposure to 28 mM SDS.