Figure 1.
Expression schematic showing directed-encapsulation of a cargo during in vivo assembly of P22. Two plasmids separately containing StAv-SP and CP, under the control of different promoters, were induced sequentially in E.coli with L-arabinose and IPTG, respectively. The resulting self-assembled P22 particles packaged the StAv (P22-StAv). This methodology, however, produced functionally inactive P22-StAv particles.