(A) Ultra wide field (UWF) fundus photograph showing multifocal neovascularization (NV), mild vitreous hemorrhage, and featureless peripheral retina.
(B) UWF fluorescein angiography (FA) confirmed NVD (white arrowhead), multiple foci of NVE (yellow arrowheads) and peripheral ischemia.
(C) SS-OCTA 12×12mm en face total retinal slab showed multifocal NV. Intraretinal microvascular abnormalities (IRMAs) (blue asterisks) were also visualized but were difficult to distinguish from preretinal NV.
(D) SS-OCTA 12×12mm en face vitreoretinal interface (VRI) slab facilitated the identification of multifocal NV and the margins of individual areas of NV. NVD is highlighted with a white arrowhead, and select areas of identifiable NVE are highlighted with yellow arrowheads, as on the corresponding FA (B). Blue asterisks mark locations where IRMAs were seen on the total retinal slab (C) but not on the VRI slab (D). The dashed yellow lines in (C) and (D) depict the location of the cross-sectional B-scans shown in (E) and (F).
(E) B-scan corresponding to the yellow line in (C) showing flow (red represents flow within retinal vessels and green represents flow within choroidal vessels) and segmentation boundaries for the total retinal slab. The slab extends from the internal limiting membrane (ILM) to the retinal pigment epithelium (boundaries shown by dotted yellow lines). NV is included within the total retinal slab because automatic segmentation algorithms do not differentiate ILM from the inner surface of NV.
(F) B-scan corresponding to the yellow line in (D) showing flow and the segmentation boundaries for the VRI slab. The slab extends from 30μm deep to the ILM to 200μm superficial to the ILM. The B-scan in (F) shows disc NV (white arrowhead) defined as preretinal vessels with detectable flow, located within the VRI slab and shown by a white arrowhead in the en face image in (D).