Fig. 2. Cysteine site-selective bioconjugation with [2.2.1]azabicyclic reagent 11. (a) Reaction between Ub-K63C and 11. General reaction conditions: Ub-K63C reacted with 11 (1 equiv.) in NaPi (20 mM, pH 7.0) at 25 °C for 30 min. (b) Deconvoluted mass spectrum obtained from the represented reaction. (c) Deconvoluted mass spectrum obtained after incubation of Ub-K63C-11 in plasma for 24 h at 37 °C.
