Skip to main content
. 2019 Apr 16;25(7-8):575–587. doi: 10.1089/ten.tea.2018.0155

FIG. 3.

FIG. 3.

ASC aggregates show a size-dependent compaction. (A) (i) Immunohistochemical staining for nuclei and F-actin. (ii) Immunohistochemical staining for E-cadherin. (iii) Hematoxylin and eosin images for 10k, 30k, and 50k (scale bar is 100 μm). (iv) Cal AM 520 staining on whole ASC aggregates (scale bar is 200 μm) (B) Nuclear compaction as determined by observation of DAPI-stained nuclear aspect ratio. (C) Volume fraction of CalAM 520 stained aggregates as calculated by imaged radius. (D) Volume fraction of F-actin and E-cadherin as calculated by imaged radius. Data represent the mean of at least three independent determinations; errors represent the standard error in the mean. ASCs, adipose-derived mesenchymal stem cells.