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. 2019 Apr 16;25(7-8):575–587. doi: 10.1089/ten.tea.2018.0155

FIG. 5.

FIG. 5.

Images of ASC size-controlled mitochondrial fission and fusion. (A) Planar and dissociated aggregate cells were stained with Mitotracker to highlight the shape of the mitochondria (scale bars are 25 μm). (B) Mitochondrial elliptical aspect ratio was calculated by dividing the major axis by the minor axis. (C) Mitochondrial circularity was calculated by dividing the area by the perimeter squared. (D) qPCR results for mitochondrial fusion genes. (E) PCR results for mitochondrial fission genes. Data represent the mean of at least three independent determinations; errors represent the standard error in the mean. *p < 0.05. PCR, polymerase chain reaction.