FIG. 1.

Overview of human testis decellularization strategy. (A) Frozen human testes were thawed, decapsulated, sliced, then agitated in trypsin/EGTA. Tissue was agitated in various detergents commonly used for decellularization to identify a preferred decellularization protocol. Differentially processed htECM was then rinsed thoroughly, disinfected by PAA, and extent of DNA removal compared with native tissue was assessed. (B) PicoGreen assay was used to quantitatively measure remnant dsDNA and compare the decellularization efficacy between detergents. Each detergent treatment removed the majority of the DNA from the native tissue, and 0.075% SDS removed the most DNA compared with other detergent treatments (*p < 0.05). Data shown as mean ± SD of technical triplicate. dsDNA, double-stranded DNA; htECM, human testicular extracellular matrix; PAA, peracetic acid; SD, standard deviation; SDS, sodium dodecyl sulfate.