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. 2019 Apr 16;25(7-8):663–676. doi: 10.1089/ten.tea.2018.0147

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Copyright 2019, Mary Ann Liebert, Inc., publishers

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FIG. 3. — Biochemical composition of ECM. (A) Alcian Blue staining indicates sGAG in htNative as well as in htECM (light blue). (B) Faint Alcian Blue staining is shown in ptNative, with no appreciable sGAG staining in ptECM. (C) Three biological replicates of human testis tissue were evaluated for sGAG content before and after decellularization. sGAG retention varies between replicates, perhaps due to biological variability between tissue donors. (D) A pooled sample of porcine testis tissue is evaluated for sGAG content; very little is measured in ptNative and none is detected in ptECM (note the axes). (E, F) Proteins from a pooled sample of human and porcine testis tissue were isolated through urea–heparin extraction, and total protein was measured by BCA assay. (G, H) bFGF was measured from the protein extractions by commercially available ELISA. Data shown as mean ± SD of technical triplicate. bFGF, basic fibroblast growth factor; sGAG, sulfated glycosaminoglycans. Color images are available online.