FIG. 2.

hADSC viability is dependent upon spheroid size and availability of metabolic substrates. (A) Representative immunofluorescence images showing TUNEL staining of hADSC spheroids after 72 h of exposure to each of the defined testing conditions. Four culture conditions were chosen to assess the effects of metabolic availability on hADSC spheroids. These conditions include Control (+Oxy/+Glu, 21% oxygen; 5.5 mM glucose), Ischemia (−Oxy/−Glu, 1% oxygen; 0.55 mM glucose), Ischemia + Glucose (−Oxy/+Glu, 1% Oxygen; 5.5 mM glucose), and Ischemia + Oxygen (+Oxy/−Glu, 21% oxygen; 0.55 mM glucose). Scale bar = 100 μm. (B) Quantification of spheroid viability after 72 h of exposure under each condition for each size group. For each culturing condition, n = 8 spheroids. ^#,##Significant difference between ischemic conditions across spheroids size groups (p < 0.05). *Significant difference between culture conditions for each spheroid size group (p < 0.05). Color images available online at www.liebertpub.com/tea