TABLE 1.
E. coli K-12-derivative strains, plasmids, and genomic islands used in this study
| Strain. plasmid, or genomic island |
Relevant genotype or phenotypea | Source or reference(s) |
|---|---|---|
| Strains | ||
| CAG18439 | MG1655 lacZU118 lacI42::Tn10 (Tc) | 54 |
| BW25113 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 | 37 |
| GG56 | Nxr derivative of BW25113 (Nx) | 55, 56 |
| VB112 | Rfr derivative of MG1655 (Rf) | 57 |
| Plasmids | ||
| pAsa4c | Cmr conjugative plasmid from A. salmonicida JF2267 (Cm) | 58 |
| pVCR94 | IncC conjugative plasmid from V. cholerae O1 El Tor (Su Tm Cm Ap Tc Sm) | 59 |
| pVCR94ΔX | Sur derivative of pVCR94, lacking the large fragment containing the multidrug resistance gene cluster | 59 |
| pVCR94Sp | Spr derivative of pVCR94ΔX (pVCR94ΔX2) (Sp Su) | 29 |
| pVCR94Kn | Knr derivative of pVCR94ΔX (pVCR94ΔX3) (Kn Su) | 29 |
| pVCR94SpΔacaCD | pVCR94Sp ΔacaCD (Sp Su) | 29 |
| pVCR94SpΔA | pVCR94Sp Δ(vcrx004-vcrx028) Δ(vcrx030-vcrx059) (Sp Su) | This study |
| pVCR94SpΔB | pVCR94Sp Δ(vcrx064-vcrx069) (Sp Su) | This study |
| pVCR94SpΔC | pVCR94Sp Δvcrx076 (Sp Su) | This study |
| pVCR94SpΔD | pVCR94Sp Δ(vcrx085-vcrx141) (Sp Su) | This study |
| pVCR94SpΔE | pVCR94Sp Δvcrx145 (Sp Su) | This study |
| pVCR94SpΔtraGC | pVCR94Sp ΔtraGC (Sp Su) | 27 |
| pBAD30 | orip15A araC PBAD (Ap) | 39 |
| peexC | pBAD30::eexC (vcrx145 of pVCR94ΔX) (Ap) | This study |
| peexCrev | pBAD30::eexCrev (vcrx145 in reverse orientation) (Ap) | This study |
| ptraGS | pBAD30::traGS (traGS from SGI1) (Ap) | 27 |
| peexC2 | pBAD30::eexC2 (YR71pYR1_0178 of pYR1) (Ap) | This study |
| peexC3 | pBAD30::eexC3 (pKHM-243_0211 of pKHM-1) (Ap) | This study |
| peexC1 | pBAD30::eexC1 (pRA1_0150 of pRA1) (Ap) | This study |
| peexD | pBAD30::eexD (sequence 139329 to 139709 of pAhD4-1) (Ap) | This study |
| peexE | pBAD30::eexE (sequence 156821 to 157174 of pAsa4c) (Ap) | This study |
| ptraGE | pBAD30::traGE (sequence 153216 to 156770 of pAsa4c) (Ap) | This study |
| pCmE | pBAD30::eexCmE (Ap) | This study |
| pEmC | pBAD30::eexEmC (Ap) | This study |
| pClo | CloDF13::TnAΔEcoRV (pSU4628) (Ap) | 60 |
| pSIM6 | Thermoinducible expression of λRed recombination (orits Ap) | 61 |
| pKD3 | cat (Cm) template for one-step chromosomal gene inactivation | 37 |
| pKD4 | aph (Kn) template for one-step chromosomal gene inactivation | 37 |
| pTT01 | tetM (Tc) template for one-step chromosomal gene inactivation | 62 |
| pE-FLP | Constitutive expression of Flp recombinase (orits Ap) | 38 |
| Genomic islands | ||
| SGI1 | SGI1 inserted into the 3′ end of trmE (Ap Cm Sp Sm Su Tc) | 29 |
| SGI1Kn | ΔIn104::aph mutant of SGI1 devoid of the integron In104 (Kn) | 27 |
| SGI1KnΔtraGS | ΔIn104::aph ΔtraGS mutant of SGI1 (Kn) | 27 |
a
Ap, ampicillin; Cm, chloramphenicol; Kn, kanamycin; Nx, nalidixic acid; Rf, rifampin; Sp, spectinomycin; Sm, streptomycin; Su, sulfamethoxazole; Tc, tetracycline; Tm, trimethoprim; ts, thermosensitive.